Zlatý kůň skull provides oldest reconstructed modern human genome over 45,000 years old. Neanderthal DNA segments longer than in Ust’-Ishim indicate she lived about 2,000 years after Neanderthal admixture. Carried typical 2-3% Neanderthal ancestry like all non-Africans. Belonged to population predating split of European and Asian ancestors. No genetic continuity with post-40,000-year-old Europeans. Early modern humans in Europe failed to contribute to today's populations. Ust’-Ishim and Oase 1 also show discontinuity. Radiocarbon dates skewed young by cow glue contamination. Skull shape similar to pre-Last Glacial Maximum Europeans. Campanian Ignimbrite eruption 39,000 years ago likely caused population turnover. Modern humans reached southeastern Europe 47-43,000 years ago. Interbreeding occurred in Near East after Out-of-Africa migration 50,000 years ago. Neanderthal segments shorten over time in modern genomes. Zlatý kůň older than or same age as Ust’-Ishim. First modern humans in Eurasia declined before non-African populations formed.