Sub-Saharan Africans exhibit marked dental homogeneity internally but differ sharply from North Africans and worldwide groups. Dental traits estimate genetic variation revealing sub-Saharans unrelated to other humans. Sub-Saharans have numerous complex crown and root traits unlike any other populations. Northeast Asians have complex sinodont teeth but sub-Saharans lack sinodont features like UI1 double shoveling. Sub-Saharan diagnostic traits include high Bushman Canine, two-rooted UP1, UM1 Carabelli's trait. Sub-Saharans show high three-rooted UM2, LM2 Y-groove, LM1 cusp 7, LP1 Tome's root. Sub-Saharans have two-rooted LM2, UM3 presence, very low UI1 double shoveling, low UM1 enamel extension. This suite forms the sub-Saharan African dental complex distinguishing them globally. Dental phenetics approximate genetics confirming sub-Saharan isolation.