Zlatý Kůň skull provides oldest sequenced modern human genome from Europe at over 45,000 years old. Genetic clock from long Neanderthal DNA segments dates her as old as or older than Siberian Ust’Ishim. She derives from early post-out-of-Africa offshoot that split before Asian-European lineages diverged. Her population shows no genetic contribution to later Eurasian hunter-gatherers or present-day Europeans. Mitochondrial DNA places her on extremely basal/old branch. Radiocarbon dates failed due to deep animal glue contamination from post-excavation preservation. Neanderthal ancestry at 3% with unusually long intact segments indicating proximity to admixture event. Lived during time Neanderthals vanished from Europe around 40,000 years ago. Campanian Ignimbrite supereruption 39,000 years ago likely decimated her group and Neanderthals. Early modern humans reached Europe over 45,000 years ago but this wave disappeared completely.